The assay can be performed on a variety of samples which can be obtained as a single cell population e.g. peripheral blood lymphocytes, nasal and buccal epithelium from clinically or occupationally exposed human population and for in vitro studies on cell lines e.g. CHO, V79, mouse lymphoma or cultured human lymphocytes and bone marrow cells. Both DNA damage and repair studies can be conducted. Also a variety of information related to genetic toxicology, human epidemiology, patients undergoing radio/chemo- therapy, ageing and nutrition can be obtained. The assay has been used for environmental biomonitoring and has utilized earthworms, fishes, mollusks exposed to polluted environments.

The migrated DNA after neutralization is stained with appropriate dye [e.g. Ethidium Bromide, Propidium Iodide, YOYO-1, Hoescht 33258, DAPI and Acridine Orange] and visualized under a fluorescent microscope. For each treatment dose / individual  50-100 cells are counted using software and both qualitative and quantitative assessment of DNA damage is carried out.

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